Contact an Account ManagerDetermination of the new HIV-protease inhibitor atazanavir by liquid chromatography after solid-phase extraction
Summary
Type: HPLC
Application #: 122350
Catalog#: 720935.40 - MN HPLC Column, Nucleosil C18 AB, 100A, 5um, 4x125mm
Detection:
Conditions: Eluent:
A) MeCN
B) 11.8 ml H3PO4 8.5% + 0.2 g sodium heptanesulfonate in 988.2 ml
H2O, pH 5.13-5.15, adjusted with NaOH 10N
C) 0.3% AcOH in MeCN
Gradient:
0. min: 15% A, 85% B, 0% C
2. min: 30% A, 70% B, 0% C
8. min: 32% A, 68% B, 0% C
18. min: 42% A, 58% B, 0% C
34. min: 46% A, 54% B, 0% C
35. min: 33% A, 34% B, 31% C
36. min: 0% A, 10% B, 90% C
37. min: 0% A, 10% B, 90% C
38. min: 33% A, 34% B, 33% C
39. min: 46% A, 54% B, 0% C
40. min: 15% A, 85% B, 0% C
47. min: 15% A, 85% B, 0% C
Flow rate: 1.0 ml/min
Temperature: RT
Injection volume: 40 µl
Authors: Colombo, S. et. al.
Source: J. Chromatogr. B 810 (2004), 25-34